Source: Bovine heart.

Purity: > 90% by SDS-PAGE, subunit apparent Mr's ~ 74,000 (E2), 53,000 (E3), 41,000 (E1a), 48,000 (E3-BP) and 36,000 (E1β). Apparent Mr ~ 8,500,000 by sedimentation equilibrium centrifugation.

Supplied: 50 μg in 50 μl 50 mM Tris-HCl pH 7.0 buffer containing 14 mM β-mercaptoethanol, 1 mM benzamidine, 0.1 mM phenylmethanesulfonyl fluoride, 1 mM EDTA and 10% glycerol. Maintain in aliquots at -70° C. Avoid repeated thawing.

Activity: ~ 12-18 μmol of NADH formed per min per mg of protein with pyruvate as substrate.

Synonyms: Pyruvate Dehydrogenase Complex.

More info: Product Datasheet

Related Products: PDC (10 μg); PDP1; BCKDH (10 μg); BCKDH (100 μg)