Source: Bovine kidney.
Purity: > 90% by SDS-PAGE, apparent Mr ~ 36-kDa.
Supplied: 50 ng (~ 1 unit) in 10 μl 50 mM Tris-HCl pH 7.0 buffer containing 14 mM β-mercaptoethanol, 0.03% Brij-35. 1 mM benzamidine, 0.1 mM phenylmethanesulfonyl fluoride, 1 mM EDTA and 65% ethyleneglycol.
Activity: ~ 17,000 units/mg with myelin basic protein (MBP) as substrate. One unit of AK is the amount of the enzyme that incorporates 1 nmol of phosphate into MBP per min. Maintain preparations in aliquots at -70° C. Avoid repeated thawing.
Synonyms: Auto-Activated Protein Kinase; Autophosphorylation-Activated Protein Kinase; Catalytic Domain of PAK2; Catalytic Domain of p21-Activated Protein Kinase.
More info: Product Datasheet
Related Products: AK/PAK2 (2 U); AK/PAK2 Assay Kit; Anti-AK/PAK2 (50 μl); Anti-AK/PAK2 (100 μl); Anti-AK/PAK2 Detection Kit; PAK1; Cdc42 (1 μg); Cdc42 (5 μg); MBP.
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