Description: Useful for determining the activity of the catalytic domain of PAK2 (AK) or related protein serine kinase. Can be used to evaluate the effect of a test substance on the activity of AK or to identify new physiological substrates of the kinase. Kit provides for 50 assays. Assay is based on the reaction of AK with myelin basic protein (MBP) and [γ-³²P]ATP. Kit includes directional insert and contains solutions of MgATP, MBP, assay and dilution buffers, and a sample of purified AK. Store solutions at -70° C. Avoid repeated thawing. [γ-³²P]ATP and trichloroacetic acid are not provided with kit.

Synonyms: Auto-Activated Protein Kinase Assay Kit; Autophosphorylation-Activated Protein Kinase Assay Kit; Assay Kit for Catalytic Domain of PAK2; Assay Kit for Catalytic Domain of p21-Activated Protein Kinase.

More info: Product Datasheet

Related Products: AK/PAK2 (1 U); AK/PAK2 (2 U); Anti-AK/PAK2 (50 μl), Anti-AK/PAK2 (100 μl); Anti-AK/PAK2 Detection Kit; PAK1; Cdc42 (1 μg); Cdc42 (5 μg); MBP.

Ref: 1. Proc Natl Acad Sci USA 90: 2500 2. J Biol Chem 268: 11193 3. J Cell Physiol 178: 397 4. J Biol Chem 276: 14909