Source: GST-tagged recombinant human produced in E. coli.

Purity: > 10% by SDS-PAGE, apparent Mr ~ 95 kDa (tagged).

Supplied: 100 ng in 50 μl 50 mM Tris-HCl pH 7.0 buffer containing 14 mM β-mercaptoethanol, 1 mM benzamidine, 0.1 mM phenylmethanesulfonyl fluoride, 1 mM EDTA, 0.03% Brij 35 and 10% glycerol.

Activity: 20 units/mg with MEK as substrate. One unit of c-raf-1 is the amount of the enzyme that generates one unit of MAP kinase per minute. One unit of MAP kinase is the amount that incorporates 1 nmol of phosphate into myelin basic protein (MBP) per min at 30° C, pH 7.0. Maintain preparations in aliquots at -70° C. Avoid repeated thawing.

Synonyms: GST-c-Raf-1; GST-cRaf1; GST-c-Raf1 protein kinase.

More info: Product Datasheet